Alogliptin (ALG) benzoate is an oral hypoglycemic drug that works as a DPP-4 inhibitor to prevent incretin degradation. Forced stability studies use high temperatures to decompose drugs and estimate their behavior at low temperatures. We aimed to evaluate the thermal stability of ALG using two techniques: isothermal thermogravimetry (TGA) and degradation in an oven followed by liquid chromatography (LC-PDA) analysis. ALG was subjected to 150, 155, 160, 165, and 170 °C up to 10% of mass loss in isothermal TGA. In the oven, the drug was submitted to 130, 140, 150, 155, 160, and 170 °C. Kinetic parameters were calculated with the Arrhenius model. ALG followed zero-order kinetics, in which the degradation rate did not depend on reagent concentration. Activation energy ranged from 31.0 to 35.9 kcal mol-1. The degraded drug was less toxic in a cytotoxicity assay in CRIB cells than the undegraded drug. The TGA method is faster and more practical than the oven followed by LC-PDA, and the data present a correlation. Here we described the kinetic parameters of ALG degradation, improving the knowledge about the drug and assisting in developing new formulations from the drug.

RESUMO JUSTIFICATIVA E OBJETIVOS: A hemofilia é uma coagulopatia congênita, rara e crônica, caracterizada pelos surtos recidivantes de hemartroses. Predomina no gênero masculino e produz agravos osteomioarticulares importantes como restrição dos movimentos, fibrose articular, hemartroses e hemorragias tissulares, contraturas musculares, déficit de força muscular e artrite hemofílica, resultando em quadros de dor e piora na qualidade de vida. O objetivo deste estudo foi verificar os efeitos de um programa fisioterápico de intervenção na dor e na qualidade de vida de um indivíduo com artrite hemofílica. RELATO DO CASO: Paciente do gênero masculino, 23 anos de idade, com diagnóstico de artrite hemofílica. Sua queixa principal era a dor em diversos pontos do corpo, especialmente em tornozelos e joelho direito. A dor foi avaliada através da escala analógica visual e do mapa de dor corporal, e a qualidade de vida por meio questionário Short-Form Health Survey. O paciente foi submetido a oito sessões de fisioterapia, uma vez por semana, com duração média de 50 minutos, entre os meses de maio a junho de 2015. O protocolo de intervenção adotado baseou-se em cinesioterapia convencional. CONCLUSÃO: O presente estudo demonstrou que um programa de fisioterapia baseado na cinesioterapia diminuiu o número de pontos dolorosos e a intensidade da dor, bem como melhorou os domínios da qualidade de vida em um indivíduo com artrite hemofílica.

ABSTRACT BACKGROUND AND OBJECTIVES: Hemophilia is a congenital, uncommon and chronic coagulopathy characterized by recurrent hemarthrosis outbreaks. It affects predominantly males and produces major musculoskeletal disorders, such as movement limitation, joint fibrosis, hemarthrosis and tissue hemorrhages, muscle contractures, muscle strength deficit and hemophilic arthritis, resulting in pain and impaired quality of life. This study aimed at evaluating the effects of a physiotherapeutic intervention program on pain and quality of life of a patient with hemophilic arthritis. CASE REPORT: Male patient, 23 years old, with hemophilic arthritis. His major complaint was pain in different body sites, especially in ankles and right knee. Pain was evaluated with the visual analog scale and body pain map, and quality of life was evaluated with the Short-Form Health Survey questionnaire. Patient was submitted to eight physiotherapy sessions, once a week, with mean duration of 50 minutes, between May and June 2015. Adopted intervention protocol was based on conventional kinesiotherapy. CONCLUSION: This study has shown that a physiotherapy program based on kinesiotherapy has decreased the number of tender points and pain intensity, as well as has improved quality of life of a patient with hemophilic arthritis.